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Simple, sensitive, and swift sequencing of complete H5N1 avian influenza virus genomes.

ABSTRACT: The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth, full-length sequence analysis. For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from H5N1 HPAIV diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument. The method presented seamlessly integrates high-throughput pyrosequencing with the Roche/454 instrument into diagnostics without the need for additional equipment or molecular biological techniques besides standard PCR and the Genome Sequencer FLX sample preparation and sequencing pipeline.

SUBMITTER: Hoper D

PROVIDER: S-EPMC2650901 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature

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Simple, sensitive, and swift sequencing of complete H5N1 avian influenza virus genomes.

Höper Dirk D Hoffmann Bernd B Beer Martin M

Journal of clinical microbiology 20081224 3

The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth, full-length sequence analysis. For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from H5N1 HPAIV diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument. The method presented seamlessly integrates high-throughput pyrosequencing with the Roche/454 instrument into diagnostics without the need for ...[more]

PMID: 19109473

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