Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR.
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ABSTRACT:
SUBMITTER: Wu J
PROVIDER: S-EPMC2650962 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Publications
Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR.
Wu Josephine J Zhang Wandi W Xie Boxun B Wu Maoxin M Tong Xiaodi X Kalpoe Jayant J Zhang David D
Journal of clinical microbiology 20081224 3
Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases. ...[more]
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