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Photoswitchable nanoparticles enable high-resolution cell imaging: PULSAR microscopy.

ABSTRACT: Beyond-diffraction-limit optical imaging of cells will reveal biological mechanisms, cellular structures, and physiological processes in nanometer scale. Harnessing the photoswitching properties of spiropyran fluorophores, we achieved nanoresolution fluorescence imaging using photoactuated unimolecular logical switching attained reconstruction (PULSAR) microscopy. The PULSAR microscope successfully resolved nanostructures and subcellular organelles when the photoswitchable nanoparticles containing spiropyran dyes were used as fluorescent probes.

SUBMITTER: Hu D 

PROVIDER: S-EPMC2651006 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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Photoswitchable nanoparticles enable high-resolution cell imaging: PULSAR microscopy.

Hu Dehong D   Tian Zhiyuan Z   Wu Wuwei W   Wan Wei W   Li Alexander D Q AD  

Journal of the American Chemical Society 20081022 46

Beyond-diffraction-limit optical imaging of cells will reveal biological mechanisms, cellular structures, and physiological processes in nanometer scale. Harnessing the photoswitching properties of spiropyran fluorophores, we achieved nanoresolution fluorescence imaging using photoactuated unimolecular logical switching attained reconstruction (PULSAR) microscopy. The PULSAR microscope successfully resolved nanostructures and subcellular organelles when the photoswitchable nanoparticles containi  ...[more]

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