High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi.
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ABSTRACT:
SUBMITTER: Holt KE
PROVIDER: S-EPMC2652037 | biostudies-literature | 2008 Aug
REPOSITORIES: biostudies-literature
Publications
High-throughput sequencing provides insights into genome variation and evolution in Salmonella Typhi.
Holt Kathryn E KE Parkhill Julian J Mazzoni Camila J CJ Roumagnac Philippe P Weill François-Xavier FX Goodhead Ian I Rance Richard R Baker Stephen S Maskell Duncan J DJ Wain John J Dolecek Christiane C Achtman Mark M Dougan Gordon G
Nature genetics 20080727 8
Isolates of Salmonella enterica serovar Typhi (Typhi), a human-restricted bacterial pathogen that causes typhoid, show limited genetic variation. We generated whole-genome sequences for 19 Typhi isolates using 454 (Roche) and Solexa (Illumina) technologies. Isolates, including the previously sequenced CT18 and Ty2 isolates, were selected to represent major nodes in the phylogenetic tree. Comparative analysis showed little evidence of purifying selection, antigenic variation or recombination betw ...[more]
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