Ontology highlight
ABSTRACT:
SUBMITTER: Seif E
PROVIDER: S-EPMC2652265 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Seif Elias E Hallberg B Martin BM
The Journal of biological chemistry 20090121 11
tRNAs that read codons starting with U are usually modified at their A37 by isopentenyl-tRNA transferases to minimize peptidyl-tRNA slippage in translation. The consensus substrate requirements of the isopentenyl-tRNA transferase of Escherichia coli, MiaA, have been the focus of extensive study. However, the molecular basis of tRNA-MiaA recognition remains unknown. Here we describe the 2.5A crystal structure of MiaA in complex with substrate tRNA(Phe). Comparative structural analysis reveals tha ...[more]