Source of phosphate in the enzymic reaction as a point of distinction among aminoglycoside 2''-phosphotransferases.

ABSTRACT: Aminoglycoside 2''-phosphotransferases are clinically important enzymes that cause high levels of resistance to aminoglycoside antibiotics by the organisms that harbor them. These enzymes phosphorylate aminoglycosides, and the modified antibiotics show significant reduction in the binding ability to target the bacterial ribosome. This report presents a detailed characterization of the antibiotic resistance profile and the aminoglycoside and nucleotide triphosphate substrate profiles of four common aminoglycoside 2''-phosphotransferases widely distributed in clinically important Gram-positive microorganisms. Although the antibiotic resistance phenotypes exhibited by these enzymes are similar, their aminoglycoside and nucleotide triphosphate substrate profiles are distinctive. Contrary to th

SUBMITTER: Toth M 

PROVIDER: S-EPMC2652286 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature