Ontology highlight
ABSTRACT:
SUBMITTER: Linford AS
PROVIDER: S-EPMC2652455 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
BMC microbiology 20090217
<h4>Background</h4>Entamoeba histolytica is an intestinal protozoan parasite of humans. The genome has been sequenced, but the study of individual gene products has been hampered by the lack of the ability to generate gene knockouts. We chose to test the use of RNA interference to knock down gene expression in Entamoeba histolytica.<h4>Results</h4>An episomal vector-based system, using the E. histolytica U6 promoter to drive expression of 29-basepair short hairpin RNAs, was developed to target p ...[more]