Ontology highlight
ABSTRACT:
SUBMITTER: Piuri M
PROVIDER: S-EPMC2654538 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Piuri Mariana M Jacobs William R WR Hatfull Graham F GF
PloS one 20090320 3
Rapid antibiotic susceptibility testing of Mycobacterium tuberculosis is of paramount importance as multiple- and extensively-drug resistant strains of M. tuberculosis emerge and spread. We describe here a virus-based assay in which fluoromycobacteriophages are used to deliver a GFP or ZsYellow fluorescent marker gene to M. tuberculosis, which can then be monitored by fluorescent detection approaches including fluorescent microscopy and flow cytometry. Pre-clinical evaluations show that addition ...[more]