Transcriptional profiling of mycobacterial antigen-induced responses in infants vaccinated with BCG at birth.

ABSTRACT:

Background

Novel tuberculosis (TB) vaccines recently tested in humans have been designed to boost immunity induced by the current vaccine, Mycobacterium bovis Bacille Calmette-Guérin (BCG). Because BCG vaccination is used extensively in infants, this population group is likely to be the first in which efficacy trials of new vaccines will be conducted. However, our understanding of the complexity of immunity to BCG in infants is inadequate, making interpretation of vaccine-induced immune responses difficult.

Methods

To better understand BCG-induced immunity, we performed gene expression profiling in five 10-week old infants routinely vaccinated with BCG at birth. RNA was extracted from 12 hour BCG-stimulated or purified protein derivative of tuberculin (PPD)-stimulated PBMC,

SUBMITTER: Fletcher HA 

PROVIDER: S-EPMC2654906 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature