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Multimeric hemicellulases facilitate biomass conversion.


ABSTRACT: Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhibit synergistic effects in hydrolysis of natural xylans and corn stover.

SUBMITTER: Fan Z 

PROVIDER: S-EPMC2655479 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature

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Multimeric hemicellulases facilitate biomass conversion.

Fan Zhanmin Z   Wagschal Kurt K   Chen Wei W   Montross Michael D MD   Lee Charles C CC   Yuan Ling L  

Applied and environmental microbiology 20090116 6


Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhibit synergistic effects in hydrolysis of natural xylans and corn stover. ...[more]

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