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Maturation of the translation inhibitor microcin C.

ABSTRACT: Microcin C (McC), an inhibitor of the growth of enteric bacteria, consists of a heptapeptide with a modified AMP residue attached to the backbone of the C-terminal aspartate through an N-acyl phosphamidate bond. Here we identify maturation intermediates produced by cells lacking individual mcc McC biosynthesis genes. We show that the products of the mccD and mccE genes are required for attachment of a 3-aminopropyl group to the phosphate of McC and that this group increases the potency of inhibition of the McC target, aspartyl-tRNA synthetase.

SUBMITTER: Metlitskaya A

PROVIDER: S-EPMC2655510 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Maturation of the translation inhibitor microcin C.

Metlitskaya Anastasia A Kazakov Teymur T Vondenhoff Gaston H GH Novikova Maria M Shashkov Alexander A Zatsepin Timofei T Semenova Ekaterina E Zaitseva Natalia N Ramensky Vasily V Van Aerschot Arthur A Severinov Konstantin K

Journal of bacteriology 20090123 7

Microcin C (McC), an inhibitor of the growth of enteric bacteria, consists of a heptapeptide with a modified AMP residue attached to the backbone of the C-terminal aspartate through an N-acyl phosphamidate bond. Here we identify maturation intermediates produced by cells lacking individual mcc McC biosynthesis genes. We show that the products of the mccD and mccE genes are required for attachment of a 3-aminopropyl group to the phosphate of McC and that this group increases the potency of inhibi ...[more]

PMID: 19168611

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Structure and organization of plasmid genes required to produce the translation inhibitor microcin C7.

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