Ontology highlight
ABSTRACT:
SUBMITTER: Kanai A
PROVIDER: S-EPMC2657004 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Kanai Akio A Sato Asako A Fukuda Yoko Y Okada Kiyoshi K Matsuda Takashi T Sakamoto Taiichi T Muto Yutaka Y Yokoyama Shigeyuki S Kawai Gota G Tomita Masaru M
RNA (New York, N.Y.) 20090120 3
Using an expression protein library of a hyperthermophilic archaeon, Pyrococcus furiosus, we identified a gene (PF0027) that encodes a protein with heat-stable cyclic nucleotide phosphodiesterase (CPDase) activity. The PF0027 gene encoded a 21-kDa protein and an amino acid sequence that showed approximately 27% identity to that of the 2'-5' tRNA ligase protein, ligT (20 kDa), from Escherichia coli. We found that the purified PF0027 protein possessed GTP-dependent RNA ligase activity and that syn ...[more]