Ontology highlight
ABSTRACT:
SUBMITTER: Criss AK
PROVIDER: S-EPMC2657081 | biostudies-literature | 2005 Oct
REPOSITORIES: biostudies-literature
Criss Alison K AK Kline Kimberly A KA Seifert H Steven HS
Molecular microbiology 20051001 2
The pilin antigenic variation (Av) system of Neisseria gonorrhoeae (Gc) mediates unidirectional DNA recombination from silent gene copies into the pilin expression locus. A DNA sequencing assay was developed to accurately measure pilin Av in a population of Gc strain FA1090 arising from a defined pilin progenitor under non-selective culture conditions. This assay employs a piliated parental Gc variant with a recA allele whose promoter is replaced by lac-regulatory elements, allowing for controll ...[more]