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Genomic characterization of novel human parechovirus type.

ABSTRACT: Using a simple metagenomic approach, we identified a divergent human parechovirus (HPeV) in the stool of a child in Pakistan. Genomic characterization showed this virus was distinct enough from reported HPeV types to qualify as candidate prototype for the seventh HPeV type.

SUBMITTER: Li L

PROVIDER: S-EPMC2657611 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Genome Sequence of Novel Human Parechovirus Type 17.

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Project description:A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has not been fixed in the paper.

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Genomic RNA folding mediates assembly of human parechovirus.

Project description:Assembly of the major viral pathogens of the Picornaviridae family is poorly understood. Human parechovirus 1 is an example of such viruses that contains 60 short regions of ordered RNA density making identical contacts with the protein shell. We show here via a combination of RNA-based systematic evolution of ligands by exponential enrichment, bioinformatics analysis and reverse genetics that these RNA segments are bound to the coat proteins in a sequence-specific manner. Disruption of either the RNA coat protein recognition motif or its contact amino acid residues is deleterious for viral assembly. The data are consistent with RNA packaging signals playing essential roles in virion assembly. Their binding sites on the coat proteins are evolutionarily conserved across the Parechovirus genus, suggesting that they represent potential broad-spectrum anti-viral targets.The mechanism underlying packaging of genomic RNA into viral particles is not well understood for human parechoviruses. Here the authors identify short RNA motifs in the parechovirus genome that bind capsid proteins, providing approximately 60 specific interactions for virion assembly.

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Complete genome sequence of a novel human parechovirus.

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