Ontology highlight
ABSTRACT:
SUBMITTER: Beau-Faller M
PROVIDER: S-EPMC2661785 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Beau-Faller M M Legrain M M Voegeli A-C AC Guérin E E Lavaux T T Ruppert A-M AM Neuville A A Massard G G Massard G G Wihlm J-M JM Quoix E E Oudet P P Gaub M P MP
British journal of cancer 20090301 6
Non-small cell lung cancers (NSCLC), in particular adenocarcinoma, are often mixed with normal cells. Therefore, low sensitivity of direct sequencing used for K-Ras mutation analysis could be inadequate in some cases. Our study focused on the possibility to increase the detection of K-Ras mutations in cases of low tumour cellularity. Besides direct sequencing, we used wild-type hybridisation probes and peptide-nucleic-acid (PNA)-mediated PCR clamping to detect mutations at codons 12 and 13, in 1 ...[more]