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Protein nanoparticles engineered to sense kinase activity in MRI.

ABSTRACT: We introduce a family of protein nanoparticles capable of sensing analytes in conjunction with magnetic resonance imaging (MRI). The new sensors are derived from the iron storage protein ferritin (Ft); they are designed and optimized using facile protein engineering methods, and self-assembled in cells harboring specific combinations of DNA coding sequences. As illustration, we show that suitably constructed Ft-based sensors can report activity of the important neural signaling enzyme protein kinase A (PKA). Phosphorylation of the engineered Ft-based nanoparticles by PKA promotes clustering and changes in T(2)-weighted MRI signal.

SUBMITTER: Shapiro MG

PROVIDER: S-EPMC2662502 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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Protein nanoparticles engineered to sense kinase activity in MRI.

Shapiro Mikhail G MG Szablowski Jerzy O JO Langer Robert R Jasanoff Alan A

Journal of the American Chemical Society 20090201 7

We introduce a family of protein nanoparticles capable of sensing analytes in conjunction with magnetic resonance imaging (MRI). The new sensors are derived from the iron storage protein ferritin (Ft); they are designed and optimized using facile protein engineering methods, and self-assembled in cells harboring specific combinations of DNA coding sequences. As illustration, we show that suitably constructed Ft-based sensors can report activity of the important neural signaling enzyme protein ki ...[more]

PMID: 19199639

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