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Mutations in three distinct loci cause resistance to peptide deformylase inhibitors in Bacillus subtilis.

ABSTRACT: Bacillus subtilis mutants with resistance against peptide deformylase inhibitors were isolated. All showed a bypass of the pathway through mutations in three genes required for formylation of Met-tRNA(fMet), fmt, folD, and glyA. glyA corresponds to a yet uncharacterized locus inducing resistance. The bypass of formylation caused robust fitness reduction but was not accompanied by alterations of the transcription profile. A subtle adaptation of the enzymes of the intermediary metabolism was observed.

SUBMITTER: Duroc Y

PROVIDER: S-EPMC2663100 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Mutations in three distinct loci cause resistance to peptide deformylase inhibitors in Bacillus subtilis.

Duroc Yann Y Giglione Carmela C Meinnel Thierry T

Antimicrobial agents and chemotherapy 20090126 4

Bacillus subtilis mutants with resistance against peptide deformylase inhibitors were isolated. All showed a bypass of the pathway through mutations in three genes required for formylation of Met-tRNA(fMet), fmt, folD, and glyA. glyA corresponds to a yet uncharacterized locus inducing resistance. The bypass of formylation caused robust fitness reduction but was not accompanied by alterations of the transcription profile. A subtle adaptation of the enzymes of the intermediary metabolism was obser ...[more]

PMID: 19171795

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