RING and coiled-coil domains of baculovirus IE2 are critical in strong activation of the cytomegalovirus major immediate-early promoter in mammalian cells.
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ABSTRACT:
SUBMITTER: Liu CY
PROVIDER: S-EPMC2663269 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Publications
RING and coiled-coil domains of baculovirus IE2 are critical in strong activation of the cytomegalovirus major immediate-early promoter in mammalian cells.
Liu Catherine Yen-Yen CY Wang Chia-Hung CH Hsiao Wen-Kai WK Lo Huei-Ru HR Wu Carol P CP Chao Yu Chan YC
Journal of virology 20090204 8
In recent years, baculovirus has emerged as a tool for high-efficiency gene transfer into mammalian cells. However, the level of gene expression is often limited by the strength of the mammalian promoter used. Here, we show that the baculovirus RING protein IE2 is a strong, promiscuous trans-activator in mammalian cells, dramatically upregulating the cytomegalovirus (CMV) promoter in both Vero E6 and U-2OS cells. Further study of the cellular mechanism for the activation led to the discovery of ...[more]
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