Ontology highlight
ABSTRACT:
SUBMITTER: Wang W
PROVIDER: S-EPMC2665785 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Wang Wei W Bradley Allan A Huang Yue Y
Genome research 20090220 4
Cultured mouse or human embryonic stem (ES) cells provide access to all of the genes required to elaborate the fundamental components and physiological systems of a mammalian cell. Chemical or insertional mutagenesis of Blm-deficient mouse ES cells can be used to generate genome-wide libraries of homozygous mutant ES cells, which are the substrates for conducting phenotype-driven loss-of-function genetic screens. However, the existing insertional mutation libraries are limited by incomplete geno ...[more]