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Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins.


ABSTRACT: Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.

SUBMITTER: Rodas C 

PROVIDER: S-EPMC2668350 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature

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Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins.

Rodas Claudia C   Iniguez Volga V   Qadri Firdausi F   Wiklund Gudrun G   Svennerholm Ann-Mari AM   Sjöling Asa A  

Journal of clinical microbiology 20090225 4


Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR. ...[more]

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