Ontology highlight
ABSTRACT:
SUBMITTER: Choi WJ
PROVIDER: S-EPMC2669314 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Choi Won Jun WJ Kim Sung-Eun SE Stephen Andrew G AG Weidlich Iwona I Giubellino Alessio A Liu Fa F Worthy Karen M KM Bindu Lakshman L Fivash Matthew J MJ Nicklaus Marc C MC Bottaro Donald P DP Fisher Robert J RJ Burke Terrence R TR
Journal of medicinal chemistry 20090301 6
A fluorescence anisotropy (FA) competition-based Shc Src homology 2 (SH2) domain-binding was established using the high affinity fluorescein isothiocyanate (FITC) containing peptide, FITC-NH-(CH2)4-CO-pY-Q-G-L-S-amide (8; Kd = 0.35 microM). Examination of a series of open-chain bis-alkenylamide containing peptides, prepared as ring-closing metathesis precursors, showed that the highest affinities were obtained by replacement of the original Gly residue with N alpha-substituted Gly (NSG) "peptoid ...[more]