Ontology highlight
ABSTRACT:
SUBMITTER: Nyabi O
PROVIDER: S-EPMC2673446 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Nyabi Omar O Naessens Michael M Haigh Katharina K Gembarska Agnieszka A Goossens Steven S Maetens Marion M De Clercq Sarah S Drogat Benjamin B Haenebalcke Lieven L Bartunkova Sonia S De Vos Ilse I De Craene Bram B Karimi Mansour M Berx Geert G Nagy Andras A Hilson Pierre P Marine Jean-Christophe JC Haigh Jody J JJ
Nucleic acids research 20090311 7
The ability to rapidly and efficiently generate reliable Cre/loxP conditional transgenic mice would greatly complement global high-throughput gene targeting initiatives aimed at identifying gene function in the mouse. We report here the generation of Cre/loxP conditional ROSA26-targeted ES cells within 3-4 weeks by using Gateway cloning to build the target vectors. The cDNA of the gene of interest can be expressed either directly by the ROSA26 promoter providing a moderate level of expression or ...[more]