Photoconversion in orange and red fluorescent proteins.
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ABSTRACT:
SUBMITTER: Kremers GJ
PROVIDER: S-EPMC2675661 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Publications
Photoconversion in orange and red fluorescent proteins.
Kremers Gert-Jan GJ Hazelwood Kristin L KL Murphy Christopher S CS Davidson Michael W MW Piston David W DW
Nature methods 20090412 5
We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date. ...[more]
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