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A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding.

ABSTRACT: The sigma factors are the key regulators of bacterial transcription initiation. Through direct read-out of promoter DNA sequence, they recruit the core RNA polymerase to sites of initiation, thereby dictating the RNA polymerase promoter-specificity. The group 1 sigma factors, which direct the vast majority of transcription initiation during log phase growth and are essential for viability, are autoregulated by an N-terminal sequence known as sigma1.1. We report the solution structure of Thermotoga maritima sigmaA sigma1.1. We additionally demonstrate by using chemical crosslinking strategies that sigma1.1 is in close proximity to the promoter recognition domains of sigmaA. We therefore propose that sigma1.1 autoinhibits promoter DNA binding of free sigmaA by stabilizing a compact organization of the sigma factor domains that is unable to bind DNA.

SUBMITTER: Schwartz EC

PROVIDER: S-EPMC2677525 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

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A full-length group 1 bacterial sigma factor adopts a compact structure incompatible with DNA binding.

Schwartz Edmund C EC Shekhtman Alexander A Dutta Kaushik K Pratt Matthew R MR Cowburn David D Darst Seth S Muir Tom W TW

Chemistry & biology 20081001 10

The sigma factors are the key regulators of bacterial transcription initiation. Through direct read-out of promoter DNA sequence, they recruit the core RNA polymerase to sites of initiation, thereby dictating the RNA polymerase promoter-specificity. The group 1 sigma factors, which direct the vast majority of transcription initiation during log phase growth and are essential for viability, are autoregulated by an N-terminal sequence known as sigma1.1. We report the solution structure of Thermoto ...[more]

PMID: 18940669

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