Ontology highlight
ABSTRACT:
SUBMITTER: Devaraj NK
PROVIDER: S-EPMC2677645 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Devaraj Neal K NK Weissleder Ralph R Hilderbrand Scott A SA
Bioconjugate chemistry 20081201 12
Bioorthogonal tetrazine cycloadditions have been applied to live cell labeling. Tetrazines react irreversibly with the strained dienophile norbornene forming dihydropyrazine products and dinitrogen. The reaction is high yielding, selective, and fast in aqueous media. Her2/neu receptors on live human breast cancer cells were targeted with a monoclonal antibody modified with a norbornene. Tetrazines conjugated to a near-infrared fluorochrome selectively and rapidly label the pretargeted antibody i ...[more]