Ontology highlight
ABSTRACT:
SUBMITTER: Strack RL
PROVIDER: S-EPMC2678115 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
BMC biotechnology 20090403
<h4>Background</h4>Whole-cell labeling is a common application of fluorescent proteins (FPs), but many red and orange FPs exhibit cytotoxicity that limits their use as whole-cell labels. Recently, a tetrameric red FP called DsRed-Express2 was engineered for enhanced solubility and was shown to be noncytotoxic in bacterial and mammalian cells. Our goal was to create derivatives of this protein with different spectral properties.<h4>Results</h4>Building on previous studies of DsRed mutants, we cre ...[more]