Ontology highlight
ABSTRACT:
SUBMITTER: Wang WW
PROVIDER: S-EPMC2678937 | biostudies-literature | 2009 Jan-Feb
REPOSITORIES: biostudies-literature
Wang Welson W WW Das Dipankar D Suresh Mavanur R MR
Molecular pharmaceutics 20090101 1
We have developed an efficient versatile in vivo dendritic cell (DC) targeting vector for delivering different classes of antigens such as proteins, peptide, glycolipids and naked DNA for vaccine applications. A single chain antibody (scFv) that recognizes DEC-205 receptor of DC was fused with a core-streptavidin domain and expressed in Escherichia coli using the T7 expression system. The bifunctional fusion protein (bfFp) was expressed as a periplasmic soluble protein and affinity-purified in i ...[more]