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Cloning and in situ expression studies of the Hydrogenobaculum arsenite oxidase genes.

ABSTRACT: Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat.

SUBMITTER: Clingenpeel SR

PROVIDER: S-EPMC2681623 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

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Cloning and in situ expression studies of the Hydrogenobaculum arsenite oxidase genes.

Clingenpeel Scott R SR D'Imperio Seth S Oduro Harry H Druschel Greg K GK McDermott Timothy R TR

Applied and environmental microbiology 20090320 10

Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat. ...[more]

PMID: 19304831

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