Rapid identification of genes encoding DNA polymerases by function-based screening of metagenomic libraries derived from glacial ice.
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ABSTRACT:
SUBMITTER: Simon C
PROVIDER: S-EPMC2681694 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
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Rapid identification of genes encoding DNA polymerases by function-based screening of metagenomic libraries derived from glacial ice.
Simon Carola C Herath Judith J Rockstroh Stephanie S Daniel Rolf R
Applied and environmental microbiology 20090306 9
Small-insert and large-insert metagenomic libraries were constructed from glacial ice of the Northern Schneeferner, which is located on the Zugspitzplatt in Germany. Subsequently, these libraries were screened for the presence of DNA polymerase-encoding genes by complementation of an Escherichia coli polA mutant. Nine novel genes encoding complete DNA polymerase I proteins or domains typical of these proteins were recovered. ...[more]
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