Integrating receptor signal inputs that influence small Rho GTPase activation dynamics at the immunological synapse.
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ABSTRACT:
SUBMITTER: Makrogianneli K
PROVIDER: S-EPMC2682016 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Publications
Integrating receptor signal inputs that influence small Rho GTPase activation dynamics at the immunological synapse.
Makrogianneli Konstantina K Carlin Leo M LM Keppler Melanie D MD Matthews Daniel R DR Ofo Enyinnaya E Coolen Anthony A Ameer-Beg Simon M SM Barber Paul R PR Vojnovic Borivoj B Ng Tony T
Molecular and cellular biology 20090323 11
The Rho GTPase Cdc42 regulates cytoskeletal changes at the immunological synapse (IS) that are critical to T-cell activation. By imaging fluorescent activity biosensors (Raichu) using fluorescence lifetime imaging microscopy, Cdc42 activation was shown to display kinetics that are conditional on the specific receptor input (through two IS-associated receptors, CD3 and beta1 integrin). CD3-triggered Cdc42 activity is dependent on the cyto-2 (NPIY) motif of the beta1 integrin cytoplasmic domain. P ...[more]
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