Ontology highlight
ABSTRACT:
SUBMITTER: Nakayasu ES
PROVIDER: S-EPMC2683718 | biostudies-literature | 2009
REPOSITORIES: biostudies-literature
Nakayasu Ernesto S ES Yashunsky Dmitry V DV Nohara Lilian L LL Torrecilhas Ana Claudia T AC Nikolaev Andrei V AV Almeida Igor C IC
Molecular systems biology 20090407
Glycosylphosphatidylinositol (GPI) anchoring is a common, relevant posttranslational modification of eukaryotic surface proteins. Here, we developed a fast, simple, and highly sensitive (high attomole-low femtomole range) method that uses liquid chromatography-tandem mass spectrometry (LC-MS(n)) for the first large-scale analysis of GPI-anchored molecules (i.e., the GPIome) of a eukaryote, Trypanosoma cruzi, the etiologic agent of Chagas disease. Our genome-wise prediction analysis revealed that ...[more]