A single C. elegans PUF protein binds RNA in multiple modes.
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ABSTRACT:
SUBMITTER: Koh YY
PROVIDER: S-EPMC2685523 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Publications
A single C. elegans PUF protein binds RNA in multiple modes.
Koh Yvonne Yiling YY Opperman Laura L Stumpf Craig C Mandan Arpita A Keles Sunduz S Wickens Marvin M
RNA (New York, N.Y.) 20090415 6
PUF proteins specifically bind mRNAs to regulate their stability and translation. Here we focus on the RNA-binding specificity of a C. elegans PUF protein, PUF-11. Our findings reveal that PUF-11 binds RNA in multiple modes, in which the protein can accommodate variable spacings between two distinct recognition elements. We propose a structural model in which flexibility in the central region of the protein enables the protein to adopt at least two distinct structures, one of which results in ba ...[more]
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