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Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.

ABSTRACT: AIM:To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine. METHODS:The esophageal squamous carcinoma cell line (TE-13) was treated with different concentrations of nicotine (100 microg/mL and 200 microg/mL) or 200 microg/mL nicotine plus 100 micromol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 expression was determined by Western blotting. Matrix metalloproteinase-2 (MMP-2) was analyzed by zymography and ELISA. RESULTS:Nicotine (100 microg/mL, 200 microg/mL) enhanced TE-13 cells migration and invasion, and increased the protein expression of COX-2 and the activity of MMP-2. Nicotine (200 microg/mL) stimulated TE-13 cells migration and invasion which were partly blocked by nimesulide. This was associated with decreased protein expression of COX-2 and decreased activity and protein expression of MMP-2. CONCLUSION:Nicotine enhances the migration and invasion of the esophageal squamous carcinoma cell line, and nimesulide partly blocks the effect of nicotine-enhanced esophageal squamous carcinoma cell migration and invasion.

SUBMITTER: Zong Y

PROVIDER: S-EPMC2686908 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature

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Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.

Zong Ye Y Zhang Shu-Tian ST Zhu Sheng-Tao ST

World journal of gastroenterology 20090501 20

<h4>Aim</h4>To study the effect of nicotine on the migration and invasion of human esophageal squamous carcinoma cells and to investigate whether nimesulide can inhibit the effect of nicotine.<h4>Methods</h4>The esophageal squamous carcinoma cell line (TE-13) was treated with different concentrations of nicotine (100 microg/mL and 200 microg/mL) or 200 microg/mL nicotine plus 100 micromol/L nimesulide. Cell migration and invasion were measured using migration and invasion chamber systems. COX-2 ...[more]

PMID: 19469000

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Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.

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Nicotine enhances migration and invasion of human esophageal squamous carcinoma cells which is inhibited by nimesulide.

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