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Fast genomic muChIP-chip from 1,000 cells.

ABSTRACT: Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (muChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. muChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.

SUBMITTER: Dahl JA

PROVIDER: S-EPMC2688267 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Fast genomic muChIP-chip from 1,000 cells.

Dahl John Arne JA Reiner Andrew H AH Collas Philippe P

Genome biology 20090210 2

Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (muChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. muChIP-chip reliably reproduces data obtained by l ...[more]

PMID: 19208222

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