Unknown

Dataset Information

0

Crystallization and preliminary X-ray analysis of the NADPH-dependent 3-quinuclidinone reductase from Rhodotorula rubra.


ABSTRACT: (R)-3-Quinuclidinol is a useful compound that is applicable to the synthesis of various pharmaceuticals. The NADPH-dependent carbonyl reductase 3-quinuclidinone reductase from Rhodotorula rubra catalyzes the stereospecific reduction of 3-quinuclidinone to (R)-3-quinuclidinol and is expected to be utilized in industrial production of this alcohol. 3-Quinuclidinone reductase from R. rubra was expressed in Escherichia coli and purified using Ni-affinity and ion-exchange column chromatography. Crystals of the protein were obtained by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitant. The crystals belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 91.3, c = 265.4 A, and diffracted X-rays to 2.2 A resolution. The asymmetric unit contained four molecules of the protein and the solvent content was 48.4%.

SUBMITTER: Takeshita D 

PROVIDER: S-EPMC2688433 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Crystallization and preliminary X-ray analysis of the NADPH-dependent 3-quinuclidinone reductase from Rhodotorula rubra.

Takeshita Daijiro D   Kataoka Michihiko M   Miyakawa Takuya T   Miyazono Ken-ichi K   Uzura Atsuko A   Nagata Koji K   Shimizu Sakayu S   Tanokura Masaru M  

Acta crystallographica. Section F, Structural biology and crystallization communications 20090523 Pt 6


(R)-3-Quinuclidinol is a useful compound that is applicable to the synthesis of various pharmaceuticals. The NADPH-dependent carbonyl reductase 3-quinuclidinone reductase from Rhodotorula rubra catalyzes the stereospecific reduction of 3-quinuclidinone to (R)-3-quinuclidinol and is expected to be utilized in industrial production of this alcohol. 3-Quinuclidinone reductase from R. rubra was expressed in Escherichia coli and purified using Ni-affinity and ion-exchange column chromatography. Cryst  ...[more]

Similar Datasets

| S-EPMC2650470 | biostudies-literature
| S-EPMC3497986 | biostudies-literature
| S-EPMC2688410 | biostudies-literature
| S-EPMC2339750 | biostudies-literature
| S-EPMC1991316 | biostudies-literature
| S-EPMC2998383 | biostudies-literature
| S-EPMC2374259 | biostudies-literature
| S-EPMC4051541 | biostudies-literature
| S-EPMC2664776 | biostudies-literature
| S-EPMC2664770 | biostudies-literature