Ontology highlight
ABSTRACT:
SUBMITTER: Kaschani F
PROVIDER: S-EPMC2689769 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Kaschani Farnusch F Gu Christian C Niessen Sherry S Hoover Heather H Cravatt Benjamin F BF van der Hoorn Renier A L RA
Molecular & cellular proteomics : MCP 20090111 5
Activity-based protein profiling is a powerful method to display enzyme activities in proteomes and provides crucial information on enzyme activity rather than protein or transcript abundance. We applied activity-based protein profiling using fluorophosphonate-based probes to display the activities of Ser hydrolases in the model plant Arabidopsis thaliana. Multidimensional protein identification technology and in-gel analysis of fluorophosphonate-labeled leaf extracts revealed over 50 Ser hydrol ...[more]