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Response of zonal chondrocytes to extracellular matrix-hydrogels.

ABSTRACT: We investigated the biological response of chondrocytes isolated from different zones of articular cartilage and their cellular behaviors in poly (ethylene glycol)-based (PEG) hydrogels containing exogenous type I collagen, hyaluronic acid (HA), or chondroitin sulfate (CS). The cellular morphology was strongly dependent on the extracellular matrix component of hydrogels. Additionally, the exogenous extracellular microenvironment affected matrix production and cartilage specific gene expression of chondrocytes from different zones. CS-based hydrogels showed the strongest response in terms of gene expression and matrix accumulation for both superficial and deep zone chondrocytes, but HA and type I collagen-based hydrogels demonstrated zonal-dependent cellular responses.

SUBMITTER: Hwang NS

PROVIDER: S-EPMC2692751 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature

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Response of zonal chondrocytes to extracellular matrix-hydrogels.

Hwang Nathaniel S NS Varghese Shyni S Lee H Janice HJ Theprungsirikul Parnduangjai P Canver Adam A Sharma Blanka B Elisseeff Jennifer J

FEBS letters 20070731 22

We investigated the biological response of chondrocytes isolated from different zones of articular cartilage and their cellular behaviors in poly (ethylene glycol)-based (PEG) hydrogels containing exogenous type I collagen, hyaluronic acid (HA), or chondroitin sulfate (CS). The cellular morphology was strongly dependent on the extracellular matrix component of hydrogels. Additionally, the exogenous extracellular microenvironment affected matrix production and cartilage specific gene expression o ...[more]

PMID: 17692846

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