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Chemical tethering of motile bacteria to silicon surfaces.

ABSTRACT: We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We expect our protocols to open new experimental doors for basic and applied studies of microorganisms, from host-pathogen relationships, to microbial forensics and drug discovery, to biosensors and biofuel cell optimization.

SUBMITTER: Bearinger J

PROVIDER: S-EPMC2693215 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature

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Chemical tethering of motile bacteria to silicon surfaces.

Bearinger Jane J Dugan Lawrence L Wu Ligang L Hill Haley H Christian Allen A Hubbell Jeffrey J

BioTechniques 20090301 3

We chemically immobilized live, motile Escherichia coli on micrometer-scale, photocatalytically patterned silicon surfaces via amine- and carboxylic acid-based chemistries. Immobilization facilitated (i) controlled positioning; (ii) high resolution cell wall imaging via atomic force microscopy (AFM); and (iii) chemical analysis with time-of-flight-secondary ion mass spectrometry (ToF-SIMS). Spinning motion of tethered bacteria, captured with fast-acquisition video, proved microbe viability. We e ...[more]

PMID: 19317664

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