Ontology highlight
ABSTRACT:
SUBMITTER: Wu S
PROVIDER: S-EPMC2693490 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Wu Si S Lourette Natacha M NM Tolić Nikola N Zhao Rui R Robinson Errol W EW Tolmachev Aleksey V AV Smith Richard D RD Pasa-Tolić Ljiljana L
Journal of proteome research 20090301 3
We present an integrated top-down and bottom-up approach that is facilitated by concurrent liquid chromatography-mass spectrometry (LC-MS) analysis and fraction collection for comprehensive high-throughput intact protein profiling. The approach employs high-resolution, reversed-phase (RP) LC separations coupled on-line with a 12 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer to profile and tentatively identify modified proteins, using detected intact protein masses in conj ...[more]