Characterization of Phase I biotransformation enzymes in coho salmon (Oncorhynchus kisutch).

ABSTRACT: Wild stocks of Pacific salmon in the Northwestern United States have declined in recent years, and the major factors contributing to these losses include water pollution and loss of habitat. In salmon, sublethal chemical exposures may impact critical behaviors (such as homing, feeding, predator-avoidance) that are important for species survival. Therefore, understanding the potential for these species to biotransform organic compounds within sensitive target tissues such as liver, gills and olfactory region can help estimate or predict their susceptibility to pollutants. In this study, we used real-time quantitative polymerase chain reaction (Q-PCR), Western blotting, and catalytic assays to characterize the expression of Phase I biotransformation enzymes in coho salmon (Oncorhynchus kisut

SUBMITTER: Matsuo AY 

PROVIDER: S-EPMC2694037 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature