Unknown

Dataset Information

0

Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein.


ABSTRACT: RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we report an efficient siRNA delivery approach that uses a peptide transduction domain-double-stranded RNA-binding domain (PTD-DRBD) fusion protein. DRBDs bind to siRNAs with high avidity, masking the siRNA's negative charge and allowing PTD-mediated cellular uptake. PTD-DRBD-delivered siRNA induced rapid RNAi in a large percentage of various primary and transformed cells, including T cells, human umbilical vein endothelial cells and human embryonic stem cells. We observed no cytotoxicity, minimal off-target transcriptional changes and no induction of innate immune responses. Thus, PTD-DRBD-mediated siRNA delivery allows efficient gene silencing in difficult-to-transfect primary cell types.

SUBMITTER: Eguchi A 

PROVIDER: S-EPMC2694965 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein.

Eguchi Akiko A   Meade Bryan R BR   Chang Yung-Chi YC   Fredrickson Craig T CT   Willert Karl K   Puri Nitin N   Dowdy Steven F SF  

Nature biotechnology 20090517 6


RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we report an efficient siRNA delivery approach that uses a peptide transduction domain-double-stranded RNA-binding domain (PTD-DRBD) fusion protein. DRBDs bind to siRNAs with high avidit  ...[more]

Similar Datasets

| S-EPMC5570165 | biostudies-literature
| S-EPMC3538314 | biostudies-literature
2009-12-31 | GSE19702 | GEO
| S-EPMC6054800 | biostudies-literature
| S-EPMC5473147 | biostudies-literature
| S-EPMC5799278 | biostudies-literature
| S-EPMC6345850 | biostudies-other
| S-EPMC6523661 | biostudies-literature
| S-EPMC4620662 | biostudies-literature
| S-EPMC3511671 | biostudies-literature