Ontology highlight
ABSTRACT:
SUBMITTER: Eguchi A
PROVIDER: S-EPMC2694965 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Eguchi Akiko A Meade Bryan R BR Chang Yung-Chi YC Fredrickson Craig T CT Willert Karl K Puri Nitin N Dowdy Steven F SF
Nature biotechnology 20090517 6
RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we report an efficient siRNA delivery approach that uses a peptide transduction domain-double-stranded RNA-binding domain (PTD-DRBD) fusion protein. DRBDs bind to siRNAs with high avidit ...[more]