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Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis.

ABSTRACT: Secondary structure content (SSC) cannot be calculated accurately from circular dichroism (CD) spectra for the majority of proteins whose three-dimensional structures have been solved. "Reliable" SSC that is significantly different from random SSC can be calculated from CD spectra only for all-alpha proteins and all-beta proteins with canonical beta-strand geometry.

SUBMITTER: Khrapunov S

PROVIDER: S-EPMC2696129 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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Circular dichroism spectroscopy has intrinsic limitations for protein secondary structure analysis.

Khrapunov Sergei S

Analytical biochemistry 20090328 2

Secondary structure content (SSC) cannot be calculated accurately from circular dichroism (CD) spectra for the majority of proteins whose three-dimensional structures have been solved. "Reliable" SSC that is significantly different from random SSC can be calculated from CD spectra only for all-alpha proteins and all-beta proteins with canonical beta-strand geometry. ...[more]

PMID: 19332020

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