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Using morpholinos for gene knockdown in Giardia intestinalis.

ABSTRACT: We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.

SUBMITTER: Carpenter ML

PROVIDER: S-EPMC2698301 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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Using morpholinos for gene knockdown in Giardia intestinalis.

Carpenter Meredith L ML Cande W Zacheus WZ

Eukaryotic cell 20090417 6

We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies. ...[more]

PMID: 19377039

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