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Molecular characterization and tissue localization of an F-box only protein from silkworm, Bombyx mori.

ABSTRACT: The eukaryotic F-box protein family is characterized by an F-box motif that has been shown to be critical for the controlled degradation of regulatory proteins. We identified a gene encoding an F-box protein from a cDNA library of silkworm pupae, which has an ORF of 1821 bp, encoding a predicted 606 amino acids. Bioinformatic analysis on the amino acid sequence shows that BmFBXO21 has a low degree of similarity to proteins from other species, and may be related to the regulation of cell-cycle progression. We have detected the expression pattern of BmFBXO21 mRNA and protein and performed immunohistochemistry at three different levels. Expression was highest in the spinning stage, and in the tissues of head, epidermis, and genital organs.

SUBMITTER: Shen Y

PROVIDER: S-EPMC2699434 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Molecular characterization and tissue localization of an F-box only protein from silkworm, Bombyx mori.

Shen Yafang Y Zhang Tiancheng T Chen Jianqing J Lv Zhengbing Z Chen Jian J Wang Dan D Nie Zuoming Z He Pingan P Wang Jiang J Zheng Qingliang Q Sheng Qing Q Wu Xiangfu X Zhang Yaozhou Y

Comparative and functional genomics 20090614

The eukaryotic F-box protein family is characterized by an F-box motif that has been shown to be critical for the controlled degradation of regulatory proteins. We identified a gene encoding an F-box protein from a cDNA library of silkworm pupae, which has an ORF of 1821 bp, encoding a predicted 606 amino acids. Bioinformatic analysis on the amino acid sequence shows that BmFBXO21 has a low degree of similarity to proteins from other species, and may be related to the regulation of cell-cycle pr ...[more]

PMID: 19557136

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Functional characterization of BmOVOs in silkworm, Bombyx mori.

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Molecular and Physiological Characterization of Two Novel Multirepeat ?-Thymosins from Silkworm, Bombyx mori.

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Subcellular localization and RNA interference of an RNA methyltransferase gene from silkworm, Bombyx mori.

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| S-EPMC2396236 | biostudies-literature

Reference Transcriptome Data in Silkworm Bombyx mori.

Project description:Herein, we performed RNA-seq analysis of ten major tissues/subparts of silkworm larvae. The sequences were mapped onto the reference genome assembly and the reference transcriptome data were successfully constructed. The reference data provided a nearly complete sequence for sericin-1, a major silk gene with a complex structure. We also markedly improved the gene model for other genes. The transcriptomic expression was investigated in each tissue and a number of transcripts were identified that were exclusively expressed in tissues such as the testis. Transcripts strongly expressed in the midgut formed tight genomic clusters, suggesting that they originated from tandem gene duplication. Transcriptional factor genes expressed in specific tissues or the silk gland subparts were also identified. We successfully constructed reference transcriptome data in the silkworm and found that a number of transcripts showed unique expression profiles. These results will facilitate basic studies on the silkworm and accelerate its applications, which will contribute to further advances in lepidopteran and entomological research as well as the practical use of these insects.

| S-EPMC8228281 | biostudies-literature

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