DNA wrapping is required for DNA damage recognition in the Escherichia coli DNA nucleotide excision repair pathway.

ABSTRACT: Localized DNA melting may provide a general strategy for recognition of the wide array of chemically and structurally diverse DNA lesions repaired by the nucleotide excision repair (NER) pathway. However, it is not clear what causes such DNA melting and how it is driven. Here, we show a DNA wrapping-melting model supported by results from dynamic monitoring of the key DNA-protein and protein-protein interactions involved in the early stages of the Escherichia coli NER process. Using an analytical technique involving capillary electrophoresis coupled with laser-induced fluorescence polarization, which combines a mobility shift assay with conformational analysis, we demonstrate that DNA wrapping around UvrB, mediated by UvrA, is an early event in the damage-recognition process during E. coli

SUBMITTER: Wang H 

PROVIDER: S-EPMC2700148 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature