Ontology highlight
ABSTRACT:
SUBMITTER: Brawley CM
PROVIDER: S-EPMC2701044 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Brawley Crista M CM Rock Ronald S RS
Proceedings of the National Academy of Sciences of the United States of America 20090528 24
Eukaryotic cells have a self-organizing cytoskeleton where motors transport cargoes along cytoskeletal tracks. To understand the sorting process, we developed a system to observe single-molecule motility in a cellular context. We followed myosin classes V, VI, and X on triton-extracted actin cytoskeletons from Drosophila S2, mammalian COS-7, and mammalian U2OS cells. We find that these cells vary considerably in their global traffic patterns. The S2 and U2OS cells have regions of actin that eith ...[more]