Unknown

Dataset Information

0

YUP.SCX: coaxing atomic models into medium resolution electron density maps.


ABSTRACT: The structures of large macromolecular complexes in different functional states can be determined by cryo-electron microscopy, which yields electron density maps of low to intermediate resolutions. The maps can be combined with high-resolution atomic structures of components of the complex, to produce a model for the complex that is more accurate than the formal resolution of the map. To this end, methods have been developed to dock atomic models into density maps rigidly or flexibly, and to refine a docked model so as to optimize the fit of the atomic model into the map. We have developed a new refinement method called YUP.SCX. The electron density map is converted into a component of the potential energy function to which terms for stereochemical restraints and volume exclusion are added. The potential energy function is then minimized (using simulated annealing) to yield a stereochemically-restrained atomic structure that fits into the electron density map optimally. We used this procedure to construct an atomic model of the 70S ribosome in the pre-accommodation state. Although some atoms are displaced by as much as 33A, they divide themselves into nearly rigid fragments along natural boundaries with smooth transitions between the fragments.

SUBMITTER: Tan RK 

PROVIDER: S-EPMC2702770 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

YUP.SCX: coaxing atomic models into medium resolution electron density maps.

Tan Robert K-Z RK   Devkota Batsal B   Harvey Stephen C SC  

Journal of structural biology 20080516 2


The structures of large macromolecular complexes in different functional states can be determined by cryo-electron microscopy, which yields electron density maps of low to intermediate resolutions. The maps can be combined with high-resolution atomic structures of components of the complex, to produce a model for the complex that is more accurate than the formal resolution of the map. To this end, methods have been developed to dock atomic models into density maps rigidly or flexibly, and to ref  ...[more]

Similar Datasets

| S-EPMC10312546 | biostudies-literature
| S-EPMC3304577 | biostudies-literature
| S-EPMC8279803 | biostudies-literature
| S-EPMC6139150 | biostudies-literature
| S-EPMC5103630 | biostudies-literature
| S-EPMC4356372 | biostudies-literature
| S-EPMC3519459 | biostudies-literature
| S-EPMC9645795 | biostudies-literature
| S-EPMC7502543 | biostudies-literature
| S-EPMC9207676 | biostudies-literature