Motif-specific sampling of phosphoproteomes.
Ontology highlight
ABSTRACT:
SUBMITTER: Ruse CI
PROVIDER: S-EPMC2703005 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Publications
Motif-specific sampling of phosphoproteomes.
Ruse Cristian I CI McClatchy Daniel B DB Lu Bingwen B Cociorva Daniel D Motoyama Akira A Park Sung Kyu SK Yates John R JR
Journal of proteome research 20080501 5
Phosphoproteomics, the targeted study of a subfraction of the proteome which is modified by phosphorylation, has become an indispensable tool to study cell signaling dynamics. We described a methodology that linked phosphoproteome and proteome analysis based on Ba2+ binding properties of amino acids. This technology selected motif-specific phosphopeptides independent of the system under analysis. MudPIT (Multidimensional Identification Technology) identified 1037 precipitated phosphopeptides fro ...[more]
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