Ontology highlight
ABSTRACT:
SUBMITTER: Guo H
PROVIDER: S-EPMC2703419 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Guo Huatao H Tse Longping V LV Barbalat Roman R Sivaamnuaiphorn Sameer S Xu Min M Doulatov Sergei S Miller Jeff F JF
Molecular cell 20080901 6
Diversity-generating retroelements (DGRs) introduce vast amounts of sequence diversity into target genes. During mutagenic homing, adenine residues are converted to random nucleotides in a unidirectional, reverse transcriptase-dependent transposition process from a donor template repeat (TR) to a recipient variable repeat (VR). Using a Bordetella bacteriophage DGR as a model, we demonstrate that homing occurs through a TR-containing RNA intermediate and is RecA independent. Marker transfer studi ...[more]