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Antenna complexes protect Photosystem I from photoinhibition.

ABSTRACT: BACKGROUND: Photosystems are composed of two moieties, a reaction center and a peripheral antenna system. In photosynthetic eukaryotes the latter system is composed of proteins belonging to Lhc family. An increasing set of evidences demonstrated how these polypeptides play a relevant physiological function in both light harvesting and photoprotection. Despite the sequence similarity between antenna proteins associated with the two Photosystems, present knowledge on their physiological role is mostly limited to complexes associated to Photosystem II. RESULTS: In this work we analyzed the physiological role of Photosystem I antenna system in Arabidopsis thaliana both in vivo and in vitro. Plants depleted in individual antenna polypeptides showed a reduced capacity for photoprotection and an increased production of reactive oxygen species upon high light exposure. In vitro experiments on isolated complexes confirmed that depletion of antenna proteins reduced the resistance of isolated Photosystem I particles to high light and that the antenna is effective in photoprotection only upon the interaction with the core complex. CONCLUSION: We show that antenna proteins play a dual role in Arabidopsis thaliana Photosystem I photoprotection: first, a Photosystem I with an intact antenna system is more resistant to high light because of a reduced production of reactive oxygen species and, second, antenna chlorophyll-proteins are the first target of high light damages. When photoprotection mechanisms become insufficient, the antenna chlorophyll proteins act as fuses: LHCI chlorophylls are degraded while the reaction center photochemical activity is maintained. Differences with respect to photoprotection strategy in Photosystem II, where the reaction center is the first target of photoinhibition, are discussed.

SUBMITTER: Alboresi A

PROVIDER: S-EPMC2704212 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Antenna complexes protect Photosystem I from photoinhibition.

Alboresi Alessandro A Ballottari Matteo M Hienerwadel Rainer R Giacometti Giorgio M GM Morosinotto Tomas T

BMC plant biology 20090609

<h4>Background</h4>Photosystems are composed of two moieties, a reaction center and a peripheral antenna system. In photosynthetic eukaryotes the latter system is composed of proteins belonging to Lhc family. An increasing set of evidences demonstrated how these polypeptides play a relevant physiological function in both light harvesting and photoprotection. Despite the sequence similarity between antenna proteins associated with the two Photosystems, present knowledge on their physiological rol ...[more]

PMID: 19508723

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Project description:Light is an important factor for determining photosynthetic performance in land plants. At high light intensity, land plants develop photosynthetic activity by increasing electron sinks, such as the Calvin cycle and photorespiration and photoprotective mechanisms in photosystem II (PSII), to effectively utilize light and protect them from photoinhibition. In addition to PSII, photosystem I (PSI) has a risk of undergoing photoinhibition under high light intensity because of the reactive oxygen species (ROS) produced within PSI. However, the acclimation response has hardly been evaluated in the relationship of PSI photoprotection to growth light. In this study, we studied the effect of growth light intensity on the photoprotective mechanisms in PSI using six wheat cultivars. To evaluate the susceptibility of PSI to its photoinhibition, we used the repetitive short-pulse (rSP) illumination method to cause O2-dependent PSI photoinhibition. We found that PSI photoinhibition induced by rSP illumination was much more alleviated in wheat cultivars grown under high-light conditions compared to those grown under low-light conditions. Here, we observed that wheat plant grown under high-light conditions lowered the susceptibility of PSI to its photoinhibition compared to those grown under low-light conditions. Furthermore, the acclimation response toward PSI photoinhibition was significantly different among the studied wheat cultivars, although the quantum yields both of PSII and PSI were increased by high-light acclimation in all wheat cultivars as reported previously. Interestingly, we observed that total chlorophyll content in leaves correlated with the susceptibility of PSI to its photoinhibition. On the basis of these results, we suggest that high-light acclimation induces protection mechanisms against PSI photoinhibition in land plants, and the increase in the leaf chlorophyll content relates to the susceptibility of PSI photoinhibition in wheat plants.

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Antenna complexes protect Photosystem I from photoinhibition.

Publications

Antenna complexes protect Photosystem I from photoinhibition.

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